Genetic and Molecular Laboratory for Training Program. (1) Dr. Zhang’s Molecular Epidemiology Lab is located at 36-051 CHS, with approximately 800 square feet. The lab has a total of 6 benches that allow 4-6 trainees to work on their wet lab projects simultaneously. The major functions of Dr. Zhang’s lab include DNA isolation, whole genome amplification, sample aliquots and plating, genotyping, and methylation assays. (2) Dr. Rao's Molecular Pathology Laboratory is located at 10-952 Factor Building, with 500 square feet. Dr. Rao’s lab is working on tissue-based assays such as immunohistochemistry (IHC), cell line-based assays, and somatic alterations. (3) The Department of Epidemiology is in the final stage of establishing a high-throughput laboratory for genetic analysis with approximately 6,300 square feet and is recruiting a director of the laboratory program. The high-throughput lab will soon be available for training purposes. In addition, many other wet labs are open for our trainees, including the Molecular and Genetic and Biomarker Core Laboratories at the UCLA Center for Human Nutrition (Dr. Zhaoping Li), the Immunology Laboratory (Dr. Otoniel Martinez-Maza), and the Carcinogenesis Laboratory at the UCLA Jonsson Comprehensive Cancer Center (Dr. Hankinson).
The UCLA Genotyping and Sequencing Core
GenoSeq, the UCLA Genotyping and Sequencing Core, is a state-of-the-art laboratory facility equipped with the latest genomic technologies. The GenoSeq Core provides services to research groups on the UCLA campus and in the broader scientific community. The Core contributes to research throughout the world, including projects from Europe, Asia, Australia, South America, and a project analyzing samples from Antarctica. Approximately 4000 scientists from over 600 academic, government, and commercial labs have used the Core since 2000. The Core is part of the UCLA David Geffen School of Medicine, whose Medical Center has been rated one of the country’s top three hospitals, and number one on the west coast, for 18 consecutive years.
The GenoSeq Core is involved in a broad range of scientific research. Medical research performed at the GenoSeq Core includes studies of human neurological disorders, such as autism, bipolar disorder, and epilepsy; cancers of breast, colon, lung, and prostate; cardiac disease; and digestive disorders. The GenoSeq Core also carries out studies of the genetics of non-human organisms including wolves in Yellowstone Park, endangered California condors, giraffes, whales, crickets, and oak. See more projects.
The DNA Microarray Core
The core can create slides with up to 15,000 genetic targets arrayed on each slide. In addition, highly parallel oligonucleotide arrays can also be fabricated to probe for mutations and sequence alterations in the genetic code. The DNA Microarray Core, which serves the whole UCLA campus, consists of high-throughput DNA arraying robots, multicolor fluorescence scanners to read the arrays, and expert staff to perform array-based experiments. The Core is directed by Professor Stanley Nelson.
Illumina Genotyping Core, Southern California Genotyping Consortium (SCGC) at UCLA
The SCGC is directed by Professor Nelson Freimer. The Core currently occupies approximately 750 square feet in the Gonda research facility on the UCLA campus. The SCGC BeadLab is equipped to perform automated Illumina GoldenGate custom genotyping assays with custom SNP pools (OPA’s) of 96, 394, 768, 1152 or 1536 SNPs. The SCGC also supports all versions of Illumina’s whole genome Infinium genotyping assay, including iSelect custom assays, methylation analysis on the meth-27 chip, and all versions of Illumina’s Gene expression chips.
Functional Proteomics Facility(Director: Dr. Joseph Loo, a Program Key Faculty). The Functional Proteomics Center at UCLA has provided state-of-the-art mass spectrometry instrumentation systems for the characterization of proteins and protein complexes, profiling protein expression, and studying post-translational modifications such as protein phosphorylation and oxidation. The facility has established a broad range of collaborative relationships including different departments at UCLA, and inter-Institute collaboration nationwide. The majority of its funding is from the NIH and its affiliates.
Research and Training Programs and Centers. High-risk study populations for population-based and hospital-based studies will be accessed by trainees through the UCLA Tumor Registry (Scientific Director: Dr. Zhang), the Center for Environmental Genomics (Drs. Schiestl and Zhang), the Molecular Toxicology Program (Dr. Hankinson), the Center for Human Nutrition (Dr. Li), and the Lung Cancer SPORE (Dr. Dubinett). In addition, trainees will have exposure and access to diverse community populations through participation in the activities of the UCLA Kaiser Permanente Center for Health Equity (Drs. Bastani and Glenn) and the Center for Cancer Prevention and Control (Drs. Ganz and Bastani).